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y of their recruitment. Importantly, immobilized RNF2 did not recruit GFP (Supplementary Figure S1F) and vice versa (data not shown), ruling out that histone ubiquitylation is caused by cross recruitment of these proteins. Previous studies have shown that RNF168 acts downstream of RNF8, as it is recruited to DSBs by binding to ubiquitin conjugates catalysed by RNF8 (Doil et al, 2009; Stewart et al, 2009).
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